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Progen Biotechnik density gradient centrifugation with lymphoprep
Density Gradient Centrifugation With Lymphoprep, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lymphoprep/lymphoprep/pm42166452-48-8-13
Average 86 stars, based on 1 article reviews
density gradient centrifugation with lymphoprep - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Isolation:

Article Title: Immunopeptidome analysis reveals SERPINB3 as an autoantigen driving eczematized psoriasis
Article Snippet: Before coculture with MODCs, T cells were stained with CFSE (Thermo Fisher Scientific, C34554 , 1 μM) according to the manufacturer’s instructions. .. PBMCs were isolated from patients’ peripheral blood using LymphoPrep (Progen Biotek, 1114547) and were magnetically separated for CD14 + cells using human CD14 MicroBeads (Miltenyi Biotec, 130-050201) with the autoMACS Pro Separator (Miltenyi Biotec, 130-092-545). .. Monocytes were then differentiated into MODCs for 7 days in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF; Miltenyi Biotec, 130-093-864, 150 U/ml) and IL-4 (Miltenyi Biotec, 130-093-920, 150 U/ml) as described previously ( ).

Article Title: Impact of Hyaluronic Acid on the Cutaneous T-Cell Lymphoma Microenvironment: A Novel Anti-Tumor Mechanism of Bexarotene.
Article Snippet: .. Human PBMCs were isolated from whole peripheral blood obtained from four HC subjects using SepMate-50 (STEMCELL Technologies, Vancouver, BC Canada) and Lymphoprep (PROGEN, Heidelberg, Germany). .. The collected PBMCs were treated with anti-CD14 microbeads (Miltenyi Biotech, Bergisch Gladbach, Germany) and positively selected using a MACS cell separation system (Miltenyi Biotech).

Article Title: Innate immune response after BNT162b2 COVID-19 vaccination associates with reactogenicity
Article Snippet: .. PBMCs were isolated from blood collected in heparin tubes using Lymphoprep (Progen) density gradient and frozen in FBS and 20 % DMSO and stored at −135 °C until use. .. Cytokines and chemokines were measured in undiluted serum (adults, i.e. aged 18 years and older) and 1:1 in PBS diluted plasma (adolescents, i.e. 12–17 years old) using a multiplex bead-based assay (LEGENDplex Human Anti-virus Response and LEGENDplex HU Proinflammatory Chemokine Panel, BioLegend) to quantify IL-1β, IL-6, IL-10, IL-12p70, IFN-α2, IFN-β, IFN-λ1, IFN-λ2/3, IFNγ, TNF-α, GM-CSF, CXCL1, CXCL5, CXCL8, CXCL9, CXCL10, CXCL11, CCL3, CCL4, CCL11, CCL17, CCL20 and MCP-1.

Article Title: Candida albicans facilitates Porphyromonas gingivalis phagocytosis and combined exposure stimulates predominantly M1 response in macrophages in vitro.
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats containing blood from healthy donors (Sanquin, Amsterdam; permission number NVT230.01), using Lymphoprep (Progen, Heidelberg, Germany) as described previously [23]. ..

Article Title: Immune signatures link myelin oligodendrocyte glycoprotein antibody-associated disease to other autoantibody-mediated conditions.
Article Snippet: Myelin oligodendrocyte glycoprotein antibody–associated disease (MOGAD) is a recently defined neurological autoimmune condition.. The pathogenesis of the disease remains poorly understood, and no specific therapies are currently approved.. Here, a comprehensive singlecell immunophenotyping of peripheral blood mononuclear cells from two independent cohorts of patients with MOGAD revealed pronounced immune perturbations in MOGAD when compared with healthy controls and patients with multiple sclerosis.

Centrifugation:

Article Title: Targeting USP14/UCHL5: A Breakthrough Approach to Overcoming Treatment-Resistant FLT3-ITD-Positive AML.
Article Snippet: .. 2024, 25, 10372 13 of 16 negative patients with AML by centrifugation using Lymphoprep (PROGEN Biotechnik GmbH, Heidelberg, Germany). ..

Article Title: Targeting USP14/UCHL5: A Breakthrough Approach to Overcoming Treatment-Resistant FLT3-ITD-Positive AML
Article Snippet: .. Specifically, the mononuclear cells were separated from the bone marrow or peripheral blood samples of four FLT3-ITD-positive and eight FLT3-ITD-negative patients with AML by centrifugation using Lymphoprep (PROGEN Biotechnik GmbH, Heidelberg, Germany). ..

Sedimentation:

Article Title: Immune signatures link myelin oligodendrocyte glycoprotein antibody-associated disease to other autoantibody-mediated conditions.
Article Snippet: Myelin oligodendrocyte glycoprotein antibody–associated disease (MOGAD) is a recently defined neurological autoimmune condition.. The pathogenesis of the disease remains poorly understood, and no specific therapies are currently approved.. Here, a comprehensive singlecell immunophenotyping of peripheral blood mononuclear cells from two independent cohorts of patients with MOGAD revealed pronounced immune perturbations in MOGAD when compared with healthy controls and patients with multiple sclerosis.



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